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Jenean Hampton was born in 1958 in inner-city Detroit, into a financially humble household. Despite watching her mother struggle to raise four girls, Jenean knew she was blessed to live in a nation where people could rise from poverty. Her mother demonstrated by example the value of hard work, and Jenean worked in banking and automotive industries to raise money for college.

After earning an Industrial Engineering degree in 1985, Jenean joined the U.S. Air Force as a computer systems officer, writing computer code and testing software. Her seven years of military service included a tour of duty in Saudi Arabia during Operation Desert Storm. After her military service, Jenean began a 19-year career in the corrugated packaging industry. She started as a crew supervisor on the production floor and was soon promoted, rising to plant manager. While working full time, Jenean earned an MBA from the University of Rochester, and moved to Bowling Green where she continued her career in the corrugated packing industry.

In 2015, she was inducted into the Kentucky Veterans Hall of Fame in recognition of both her military service to the nation and general community service during her post-military career.

Jenean is married to retired U.S. Air Force Colonel Dr. Doyle Isaak. They reside in Bowling Green, where she is active in the community and in political/civic arenas. She is a member of many local organizations including American Legion Post 23, the Civil Air Patrol, and the Bowling Green Women's Club. On November 3, 2015, Jenean was elected Lt. Governor of Kentucky and became the first African-American to hold statewide office.